BIOL 425 - Techniques in Mammalian Cell Culture

Units: 3
Techniques of in vitro culturing of primary and established cell lines of multi-cellular origin. Topics include nutrition, growth, cloning, cell fusion, transformation, preservation, karyotyping, autoradiography, metabolic labeling, quality control applications. Lecture Units: 1; Lab Units: 2

Prerequisites: BIOL 310 and BIOL 330.
Equivalent Quarter Course: BIOL 4450.
Possible Instructional Methods: Entirely On-ground.
Grading: A-F grading only.
Course Typically Offered: Spring ONLY

Student Learning Outcomes - Upon successful completion of this course students will be able to:

apply appropriate cell culture techniques, following safe procedures and adequate control of facilities, equipment, and reagents;
apply qualitative methods of mammalian cell cultures, i.e., cell counting and analysis;
demonstrate appropriate cryopreservation and cell banking techniques;
apply methods related to sterility and microbial contamination tests;
gather, interpret, and evaluate published scientific information involving mammalian cell culture;
interpret data from experiments using the relevant methodologies described and draw appropriate conclusions from these data;
maintain and manipulate primary and established cell lines under various experimental conditions (e.g., mitogenic stimulation and exposure to cellular stresses);
create transient and stable transfection of mammalian cells, expressing wild type and mutant proteins;
conduct immunofluorescent analysis of subcellular localization of transiently expressed proteins, as well as assess the viability and proliferation of cells grown under various experimental conditions using multiple proliferation assays;
create and maintain a professional laboratory notebook.