CHEM 443 - Biochemistry Laboratory I

Units: 3
Laboratory techniques in biochemistry with a focus on protein purification and enzyme kinetics. Intended to supplement CHEM 441 Biochemistry I. Lecture Units: 1; Lab Units: 2.

Strongly Recommended Preparation: CHEM 220.
Co-requisites: CHEM 441.
Possible Instructional Methods: Entirely On-ground.
Grading: A-F grading only.
Course Typically Offered: Fall ONLY

Student Learning Outcomes - Upon successful completion of this course students will be able to:

Apply buffer theory and prepare a laboratory buffer;
Conduct protein and enzyme activity assays and calculate results from laboratory-derived data;
Collect and analyze enzyme kinetic data and present this data in graphical format;
Calculate and interpret data commonly found in protein purification tables;
Summarize the principles of various chromatographic methods and outline the practical procedures;
Explain the theory of protein electrophoresis and utilize this technique in laboratory experimentation;
Develop and maintain an informative and up-to-date laboratory notebook and compose well-written laboratory reports.